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human set2 leukemia cell line with jak2 v617f mutation  (DSMZ)


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    DSMZ human set2 leukemia cell line with jak2 v617f mutation
    FDCP-EpoRV617F cells were exposed to 1 μm atiprimod alone (control) or in the presence of HS5 stromal cells (upper panel) or NK.tert stromal cells (lower panel) for 4, 24, and 48 hours. Cells were then lysed and whole cell lysates were immunoprecipitated with a rabbit <t>anti-JAK2</t> antibody for detection of p-JAK2. Western blot analysis using anti-phosphotyrosine antibody was performed. Then, membranes were stripped and reprobed with anti-JAK2.
    Human Set2 Leukemia Cell Line With Jak2 V617f Mutation, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+set2+leukemia+cell+line+with+jak2+v617f+mutation/set+2+cells/pmc04067142-48-6-12
    Average 90 stars, based on 1 article reviews
    human set2 leukemia cell line with jak2 v617f mutation - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR"

    Article Title: BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR

    Journal: Cancer research

    doi: 10.1158/0008-5472.CAN-10-4002

    FDCP-EpoRV617F cells were exposed to 1 μm atiprimod alone (control) or in the presence of HS5 stromal cells (upper panel) or NK.tert stromal cells (lower panel) for 4, 24, and 48 hours. Cells were then lysed and whole cell lysates were immunoprecipitated with a rabbit anti-JAK2 antibody for detection of p-JAK2. Western blot analysis using anti-phosphotyrosine antibody was performed. Then, membranes were stripped and reprobed with anti-JAK2.
    Figure Legend Snippet: FDCP-EpoRV617F cells were exposed to 1 μm atiprimod alone (control) or in the presence of HS5 stromal cells (upper panel) or NK.tert stromal cells (lower panel) for 4, 24, and 48 hours. Cells were then lysed and whole cell lysates were immunoprecipitated with a rabbit anti-JAK2 antibody for detection of p-JAK2. Western blot analysis using anti-phosphotyrosine antibody was performed. Then, membranes were stripped and reprobed with anti-JAK2.

    Techniques Used: Control, Immunoprecipitation, Western Blot

    Heat map depicting the changes in levels of 27 secreted cytokines after 4, 24, and 48 hours of co-culture (stromal cells and JAK2-mutation positive cells separated by membrane) with or without atiprimod. Rows, cytokines; columns, profiled samples. Yellow, high expression; blue, low expression; black, no change. Each treated sample is centered on the corresponding control sample from the same time point. Cytokines chosen for further functional studies are highlighted (bold italics).
    Figure Legend Snippet: Heat map depicting the changes in levels of 27 secreted cytokines after 4, 24, and 48 hours of co-culture (stromal cells and JAK2-mutation positive cells separated by membrane) with or without atiprimod. Rows, cytokines; columns, profiled samples. Yellow, high expression; blue, low expression; black, no change. Each treated sample is centered on the corresponding control sample from the same time point. Cytokines chosen for further functional studies are highlighted (bold italics).

    Techniques Used: Co-Culture Assay, Mutagenesis, Membrane, Expressing, Control, Functional Assay

    Related Articles

    Mutagenesis:

    Article Title: BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR
    Article Snippet: .. Human SET2 leukemia cell line with JAK2 V617F mutation was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig, Germany), and maintained in RPMI1640 medium supplemented with 20% FCS. .. Human stromal NK.tert cell line (derived from human bone marrow cells immortalized with hTERT) containing exogene MFG-tsT-IRES-neo was obtained from the RIKEN Cell Bank (Sapporo Medical University, Japan)( 12 ) and cultured in alpha-Minimum Essential Medium Eagle with Earl salts and L-glutamine (α-MEM; Invitrogen) supplemented with 12.5% FCS (HyClone), 12.5% human serum (Cellgro), 1 μm hydrocortisone (Sigma-Aldrich), and 100 μm 2-mercaptoethanol (Sigma-Aldrich).



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    human set2 leukemia cell line with jak2 v617f mutation  (DSMZ)
    90
    DSMZ human set2 leukemia cell line with jak2 v617f mutation
    FDCP-EpoRV617F cells were exposed to 1 μm atiprimod alone (control) or in the presence of HS5 stromal cells (upper panel) or NK.tert stromal cells (lower panel) for 4, 24, and 48 hours. Cells were then lysed and whole cell lysates were immunoprecipitated with a rabbit <t>anti-JAK2</t> antibody for detection of p-JAK2. Western blot analysis using anti-phosphotyrosine antibody was performed. Then, membranes were stripped and reprobed with anti-JAK2.
    Human Set2 Leukemia Cell Line With Jak2 V617f Mutation, supplied by DSMZ, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+set2+leukemia+cell+line+with+jak2+v617f+mutation/set+2+cells/pmc04067142-48-6-12
    Average 90 stars, based on 1 article reviews
    human set2 leukemia cell line with jak2 v617f mutation - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    FDCP-EpoRV617F cells were exposed to 1 μm atiprimod alone (control) or in the presence of HS5 stromal cells (upper panel) or NK.tert stromal cells (lower panel) for 4, 24, and 48 hours. Cells were then lysed and whole cell lysates were immunoprecipitated with a rabbit anti-JAK2 antibody for detection of p-JAK2. Western blot analysis using anti-phosphotyrosine antibody was performed. Then, membranes were stripped and reprobed with anti-JAK2.

    Journal: Cancer research

    Article Title: BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR

    doi: 10.1158/0008-5472.CAN-10-4002

    Figure Lengend Snippet: FDCP-EpoRV617F cells were exposed to 1 μm atiprimod alone (control) or in the presence of HS5 stromal cells (upper panel) or NK.tert stromal cells (lower panel) for 4, 24, and 48 hours. Cells were then lysed and whole cell lysates were immunoprecipitated with a rabbit anti-JAK2 antibody for detection of p-JAK2. Western blot analysis using anti-phosphotyrosine antibody was performed. Then, membranes were stripped and reprobed with anti-JAK2.

    Article Snippet: Human SET2 leukemia cell line with JAK2 V617F mutation was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig, Germany), and maintained in RPMI1640 medium supplemented with 20% FCS.

    Techniques: Control, Immunoprecipitation, Western Blot

    Heat map depicting the changes in levels of 27 secreted cytokines after 4, 24, and 48 hours of co-culture (stromal cells and JAK2-mutation positive cells separated by membrane) with or without atiprimod. Rows, cytokines; columns, profiled samples. Yellow, high expression; blue, low expression; black, no change. Each treated sample is centered on the corresponding control sample from the same time point. Cytokines chosen for further functional studies are highlighted (bold italics).

    Journal: Cancer research

    Article Title: BONE MARROW STROMA SECRETED CYTOKINES PROTECT JAK2 V617F -MUTATED CELLS FROM THE EFFECTS OF A JAK2 INHIBITOR

    doi: 10.1158/0008-5472.CAN-10-4002

    Figure Lengend Snippet: Heat map depicting the changes in levels of 27 secreted cytokines after 4, 24, and 48 hours of co-culture (stromal cells and JAK2-mutation positive cells separated by membrane) with or without atiprimod. Rows, cytokines; columns, profiled samples. Yellow, high expression; blue, low expression; black, no change. Each treated sample is centered on the corresponding control sample from the same time point. Cytokines chosen for further functional studies are highlighted (bold italics).

    Article Snippet: Human SET2 leukemia cell line with JAK2 V617F mutation was purchased from DSMZ (Deutsche Sammlung von Mikroorganismen und Zellkulturen Braunschweig, Germany), and maintained in RPMI1640 medium supplemented with 20% FCS.

    Techniques: Co-Culture Assay, Mutagenesis, Membrane, Expressing, Control, Functional Assay